- Breaks down the α-1,6 glycosidic bonds of pulullan, branched-chain starches and corresponding oligosaccharides.
- It forms straight-chain dextrins and improves the rate and utilization of starch breakdown.
Pullulanase is used in combination with other enzymes (glycosylase, β-amylase, fungal α-amylase). This can speed up the reaction and increase the glucose and maltose content.